Estabishing a new greenhouse experiment with simulated petroleum contaminated soil

The main goals of the greenhouse experiment are: to access the resistance of Miscanthusxgiganteus while growing at the simulated petroleum contaminated soil and to determine the limited level of soil petroleum contamination when the plant develops well and produces a good biomass.

The soil used for the experiment was taken at the petroleum refinery (Microchem company, Trebon, Czech Republic) and was polluted by different hydrocarbons. That soil was diluted with Mimon soil taken at the military site 1 (2016) which was not polluted by hydrocarbons. The different ratios of petroleum polluted-not polluted soils (0:1, 1:2, 1:1, 2:1, 1:0) were used for the experiment.

4 parallel pots with 6 kg of soil in each were prepared for each selected ratio. M.xgiganteus rhizomes were planted in 3 pots and one control pot was left without rhizomes. The rhizomes used for planting were originally from the same plant in order to ensure a genetic consistency and to avoid the effect of different stress tolerance.

The impact of soil petroleum contamination to plant’s biometric parameters, stress response and structure of soil microbial communities will be monitored during vegetation season.

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1_collecting of clean soil in Hradüany airport
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2_clean soil and contaminated soil from previous experiment
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3_prepared pots
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4_Miscanthus plants after 17 days

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