Publications

Publications NATO SPS MYR G4687

(project started on April 28th, 2016; active starting day- October 6,2016)

Articles

1.Pidlisnyuk V., Trogl J., Stefanovska T., Shapoval P., Erickson L.(2016). Preliminary results on growing second generation biofuel crop Miscanthusxgiganteus at the polluted military site in Ukraine. Nova Biotechnologica et Chimica, 15-1, p.77-84. DOI: 10.1515/nbec-2016-0008 ( IF=0,188).

The semi-field research on exploring opportunity to use second-generation biofuel crop Miscanthus x giganteus for restoration of former military site in Kamenetz-Podilsky, Ukraine was carried out during two vegetation seasons. Despite high metal pollution of soil, in particular, by Fe, Mn, Ti, and Zr, no growth inhibition was observed. The concentrations followed pattern soil > roots > stems > leaves. For some metals as As and Pb the concentration in the above-ground parts were statistically comparable to zero. Generally, no correlations was observed between concentrations of metals is the soil and in the upper plant’s parts. The research confirmed the ability of Miscanthus x giganteus to grow on the military soils predominantly contaminated by heavy metals.

2.Sailaukhanuly Y., Carlsen L., Tulegenov A., Nurzhanova A, Kenessov B., Kamysbayev D. (2016) Distribution and risk assessment of selected organochlorine pesticides in Kyzyl Kairat village from Kazakhstan. Environmental Monitoring and Assessment, 188:358 DOI: 10.1007/s10661-016-5353-9 (IF=1.633)

Concentrations of selected organochlorine pesticides (OCPs), i.e.,4,4’-dichlorodiphenyltrichloroethane (p,p’-DDT), its metabolites (p,p’-DDE, p,p’-DDD), and hexachlorocyclohexanes (HCHs) have been determined in 100 soil samples collected from a contaminated site centered around a former storehouse in the Kyzyl Kairat village, Almaty region, Kazakhstan, that constitutes an exemplary case example. The OCPs were observed in all analyzed soil samples, with predominance of α-HCH, p,p′-DDD, p,p′-DDE and p,p′-DDT. Total concentrations ranged from 1.38 to 11,100 µg kg-1 with an average value of 1,040 µg kg-1 for DDT and its metabolites, and 0.1 to 438 µg kg-1 with an average value of 24 µg kg-1 for HCHs. The observed concentrations of the OCPs were found to be in agreement with previous studies and are rationalized in terms of the possible degradation pathways of DDTs and HCHs. Spatial distribution patterns of OCPs are elucidated by contour maps. Observed concentrations of the OCPs were used to evaluate the cancer risk to humans via ingestion, dermal contact and inhalation of soil particles. The cancer risk mainly occurs from ingestion, whereas dermal exposure contributes to a minor extent to the total cancer risk. The risk associated with inhalation was found to be negligible. The total cancer risk for the studied OCPs were found to be: p,p’-DDT ˃ p,p’-DDE ˃ p,p’-DDD ˃ α-HCH ˃ β-HCH ˃ γ-HCH.

Proceeding

1. Pidlisnyuk V., Shapoval P., Stefanovska T., Hettiarachchi G., Erickson L., Davis D., Trogl J. (2016) Recovering of soil from the military contaminated site in Sliac, Slovakia by Miscanthus x guganteus. Abstract Book. International multidisciplinary conference "Sustainable technologies and the legal/economic aspects of agricultural production" 27-28 April 2016, Kyiv, Ukraine, 109-111.

2. Nurzhanova A., Pidlisnyuk V., Sailaukhanyly Y., Nurzhanov Ch., Naizabayeva L., Kalugin S., Stefanovska T. (2016) Physiological basis of the stability of miscanthus to soils polluted by heavy metals // Abstract Book. International multidisciplinary conference "Sustainable technologies and the legal/economic aspects of agricultural production" 27-28 April 2016, Kyiv, Ukraine, 115-119.

3. Stefanovska T., Pidlisnyuk V., Khabalikyan A., Yaschuk S., Ovruch M. (2016) The impact of Miscanthus x giganteus cultivation on interebrate diversity in agricultural landscapes. International multidisciolinary conference « Sustainable technologies and the legal/economic aspects of agricultural production», 27-28 April, 2016, NULES, Kyiv, Ukraine, 112-113.

4. Stefanovska T., Kucherovska S., Lewis E., Pidlisnyuk V. (2016) Enthomocomplex of energetic plants for production of second generation biofuels.International multidisciolinary conference « Sustainable technologies and the legal/economic aspects of agricultural production», 27-28 April, 2016, NULES, Kyiv, Ukraine, 107-109.

5. Pidlisnyuk V., Trogl J., Stefanovska T. (2016) Effect of growing biofuel crop Miscanthusxgiganteus at the military contaminated soil to the microbial communities’ characteristics .Eurosoil, Istanbul 2016, 17-22 July-TEMMUZ 2016, report L-490.

6. Stefanovska T., Pidlisnyuk V.(2016) Using nematodes for determination state of phizosphere in phytotechnology with miscanthus x giganteus implemented for military heavy metal contaminated soil in Ukraine.Eurosoil, Istanbul 2016, 17-22 July-TEMMUZ 2016, and report V-390.

7. Pidlisnyuk V., Stefanovska T., Shapoval P.(2016) Developing perennial phytotechnology for contaminated military site: case of Kamenetz-Podilsky, Ukraine. Proceeding of the International conference Contaminated Sites, Bratislava, Slovakia, September 12-13, 2016, 124-132.

Manuscripts submitted for publication

Stefanovska T., Pidisnyuk V., Lewis E., Gorgatenko A., Herbivorous insects diversity at Miscanthusxgigantes in Ukraine. Submitted to the Journal for Agricultural Sciences, March, 2017.

Publications, NATO SPS Planning Grant #984687
1. Patent

Pidlisnyuk V, Stefanovska T. (2014) Methods for growing plants in heavy metals contaminated soils. Claim for the Invention of Ukraine, 2013, #12471
Letter #2413/3A/14 from State Intellectual Service of Ukraine, January 29, 2014

2. Articles

1.Pidlisnyuk V., Erickson L., Kharchenko S., Stefanovska T. (2014) Sustainable Land Management: Growing Miscanthus in Soils contaminated with heavy Metals”. Journal of Environmental Protection, Special Issue in Environmental Remediation, 5, 723-730. Published Online June 2014 in SciRes. http://www.scirp.org/journal/jep
http://dx.doi.org/10.4236/jep.2014.58073
The article presents the results of a study about the first model experiments in greenhouses were carried out to determine the possibility of using M.xgiganteus in the phytoremediation of contaminated soils with heavy metals.

2.Nurzhanova A., Pidlisnyuk V., Sailaukhanyly Y., Kalugin S., Mursalieva V., Stefanovska T., Erickson L. (2015) Miscanthus x giganteus – promising bioenergetic phytoremediant. Reports of the National Academy of Sciences of the Republic of Kazakhstan 303, 128 – 138 (in Russian).

The article analyzes the possibility of using highly productive bioenergetics phytoremediant
Miscanthus x giganteus, having an ability to grow on marginal and degraded of anthropogenic pollutants lands. It is of highly productive, non-food perennial plant with a high content of lignin and cellulose in stem. It is used in many countries for phytostabilisation polluted soils with persistent xenobiotics. The high productivity of biomass M.xgiganteus on the contaminated degraded lands can turn the phytoremediation technology into a profitable bioenergy industry in our country.

3.Nurzhanova A., Pidlisnyuk V., Kalugin S., Stefanovska T., Drimal M. (2015) Miscanthus x giganteus as a new highly efficient phytoremediaion agent for improving soils contaminated by pesticidies reqidues and supplemented contaminants. Communications in Applied Biological Sciences, 80/3,  361-366 (IF=0,09)

Soil monitoring was accomplished at 76 former pesticide storehouses in Kazakhstan. Pesticides’ gas chromatography analysis was limited to the organochlorine pesticides DDT (p,p'-dichlorodiphenyltrichloroethane) and HCH (hexachlorocyclohexane), and their associated metabolites and isomers: 2.4’DDD (p,p'-dichlorodiphenyl dichloroethane); 4.4’DDD; 4.4’DDT; 4.4’DDE(p,p'-dichlorodiphenyldichloroethylene); α-HCH; β-HCH; and γ-HCH. The studies showed exceeding of Maximum Acceptable Concentration (MAC) by 10 times at soil samples taken at 24 former pesticide storehouses, and the basic pollutants were isomers of α HCH, β HCH and metabolite of 4.4’DDE, 4.4’DDT, supplemented by heavy metals. Monitoring data demonstrated the potential ecological danger and health risk posed by the sites, especially those located near populated areas. In order to eliminate the negative environmental and health effect it was proposed to use phytotechnology with second generation biofuel crop Miscanthus x giganteus. The technology applied directly at the contaminated area (in situ), helping to decrease costs and to reduce exposure from polluted sites. Plant shows good growing at the soil contaminated by pesticides during vegetation season.

4. Nurzhanovа, А. Nurmagambetova, R. Zhamanbalinova, А. Balmukanov, E. Sailaukhanuly Physiological and biochemical characteristics of Miscanthus x giganteus in polluted conditions // Reports of the National Academy of Sciences of the Republic of Kazakhstan. Series of biological and medical – 2016. – V. 1, N. 313 – P. 102 – 109 (in Russian)

The article presents the results of a study of the basic adaptive physiological and biochemical indicators of the species of second-generation biofuel Miscanthus x giganteus of the stability to heavy metals. It is shown that the water absorption capacity, a change in the ratio of the concentration of chlorophyll a concentration of carotenoids, the activity of free proline in leaves are the main indicators of adaptive of the assimilation apparatus to stress. It was revealed that the species M.giganteus has phytoremediation potential and refers to excluders in relation to the heavy metals

Proceedings

1. Kucherovska S., Stefanovska T., Pidlisnyuk V.(2014)Environmental security during growing of second generation of biofuel crops: case of perennial Panicum virgatum L. Proceedings of the 4th CEECHE conference, May 25-30, 2014, Cluj-Napoka, Rumania

2.Tatarina N., Pidlisnyuk V., Stefanovska T., Erickson L. (2014)Phytoremediation of metal-contaminated soils in Kamenetz-Podilsky, Ukraine by growing of second generation crop Miscanthus x giganteus// CEECHE conference, May 25-30, 2014, Cluj-Napoka, Rumania

3.Davis L. C. , Erickson L. E. , Hettiarachchi G., Mengarelli J., Pidlisnyuk V. , Roozeboom K. , Stefanovska T. , Tatarina N.(2014). Phytoremediation with Miscanthus produced for bioenergy. Proceeding of the 11th International Phytotechnologies conference, Heraklion, Crete, Greece, 30.09-3.10.2014, Book of Abstracts, edited by N.Kalogerakis and Th.Manios, p.313.ISBN 978-960-6865-81-7

4.Stefanovska T., Kucherovska S., Pidlisnyuk V. (2014)Assessing of Herbivorous and beneficial insects on Switchgrass Panium Virgatum in Ukraine. Proceeding of the 66th International Symposium on Crop Protection, Gent, Belgium, 20.05.2014, p.114

5. Pidlisnyuk V., Stefanovska T., Erickson L., Drimal M., Tomashkin J., Shapoval P., Koval N. (2015).New phytotechnology for cleaning contaminated military sites in Slovakia and Ukraine. Conference proceedings. International conference Contaminated sites. Bratislava 27-May 29, 2015. p. 135-139. Polygraph Plus s.r.o, Banska Bystrica, Slovakia. ISBN 978-80-89503-40-7

6. Nurzhanova A., Pidlisnyuk V., Kalugin S., Stefanovska T., Drimal M. (2015) Miscantus xgiganteus as a new highly effecient phytoremediation agent for improving soils contaminated by persticides residues and supplemented contaminants.Abstracts of the 67th International conference in Plant protection, Gent University, Faculty of Bioscience engineering. May,19th, 2015. p.151. at:www.iscp_ugent.be/pdf/book_of_abstracts_2015.pdf

7.Pidlisnyuk V., Stefanovska T., Erickson L., Shapoval P., Koval N., Davis L. (2015) Perennial phytotechnology for sustainable land management at the former military sites in Slovakia and Ukraine. 12 International Phytotechnology conference, Kansas, USA .12 International Phytotechnology Conference “Phytotechnologies for Sustainable Development” , September 27-30.2015, Manhattan, Kansas, USA. Abstract Book, Editor Larry E.Erickson, ISBN 178-0-692-50825-1.p.32

8.Stefanovska T., Pidlisnyuk V., Erickson L., Dolya M., Lewis E., Vystavna Y. (2015). Potential to evaluate the efficiancy of phytotechnology with Miscanthus x giganteus at the abandoned military sites by using soil nematodes as bioindicators.12 International Phytotechnology Conference “Phytotechnologies for Sustainable Development” , September 27-30.2015, Manhattan, Kansas, USA. Abstract Book, Editor Larry E.Erickson, ISBN 178-0-692-50825-1.

9. Stefanovska T., Pidlisnyuk V., Lewis E. (2016) Assessment of phytotechnology efficiancy by using the soil nematode community structure as bioindicators. Abstract Book. CEECHE 2016 (Central and Easter European Conference on Health and the Environment), April 10-14, Prague, Czech Republic, P.48, p.125

10. Pidlisnyuk V., Nurzhanova A., Stefanovska T.,Erickson L. (2016) Phytoremediation of soil contaminated by xenobiotic pollutants by using elephant grass Miscanthusxgiganteus and switchgrass Panicum virgatum L. // Abstract Book. CEECHE 2016 (Central and Easter European Conference on Health and the Environment), April 10-14, Prague, Czech Republic, P50, p.127.

11. Pidlisnyuk V., Stefanovska T., Trogl J.,Jilkova J., Shapoval P., Kharchenko M., Erickson L., Hettiarachchi G., Davis L. (2016) Prevention exposure from contaminated military sites through phytoremediation with second generation biofuel crop Miscanthusx giganteus. Abstract Book. CEECHE 2016 (Central and Easter European Conference on Health and the Environment), April 10-14, Prague, Czech Republic, P49, p. 126

Draft Book Plan (as for March, 2017)

Book Title



Phytoremediation with Biomass Production: Improving Soil Quality for Sustainable Development



Chapter 1.  Soil Quality in the World Today


1.1.  Characteristics of fertile and healthy soils


1.2.  Characteristics of contaminated soils


1.3.  Types of contaminants in soils


1.4.  Extent of soil ecosystem degradation

Chapter

2.  Phytotechnologies for Soil Remediation


2.1.  Phytoremediation


2.2.  Phytovolatilization


2.3.  Phytostabilization


2.4.  Phytoremediation with biomass production



Chapter 3  Establishing Vegetation in Contaminated Soil


3.1. Site characterization


3.2. Soil amendments


3.3. Plant nutrition


3.4. Contaminant toxicity


3.5. Plant selection


3.6. Microbial ecology

Chapter

4.  Production of Biomass in Contaminated Soils


4.1.  Micanthus


4.2.  Switchgrass


4.3.  Trees

Chapter

5.  Improving Soil Quality


5.1.  Adding organic matter


5.2.  Improving biological quality and soil ecology


5.3.  Adding plant nutrients



Chapter 6.  Soil Water Holding Capacity


6.1.  Water in unsaturated soil


6.2.  Improving water holding capacity


6.3.  Organic matter



Chapter 7.  Metals in Contaminated Soil


7.1.  Phytostabilization and bioavailability


7.2.  Production of energy crops


7.2.1. Miscanthus


7.2.2. Switchgrass


7.2.3. Trees


7.3.  Organic matter


7.4.  Microbial ecology



Chapter 8. Organic Contaminants in Soils


8.1.  Bioremediation and biodegradation


8.2.  Phytoremediation


8.3.  Phytoremediation with biomass production



Chapter 9. Phytoremediation and Bioremediation of Pesticide-Contaminated Soil


9.1   Soils contaminated with pesticides


9.2   Soil ecology


9.3   Methods of analysis


9.4   Remediation alternatives


9.5   Types of pesticides


9.6   Implementing and monitoring phytoremediation



Chapter 10.  Markets for Miscanthus and Other Biomass Products


10.1.  Bioenergy products


10.2.  Animal bedding


10.3.  Building materials


10.4.  Other products



Chapter 11.  Pest Management and Soil Ecology


11.1.  Miscanthus


11.2.  Switchgrass


11.3.  Trees



Chapter 12.  Soil Carbon


12.1.  Adding carbon to soils


12.2.  Benefits of soil carbon


12.3   Impact on global carbon balance



Chapter 13. Conclusions
Reports delivered at the international conferences and meeting

1. Pidlisnyuk V.(presenting author), Shapoval P., Stefanovska T., Hettiarachchi G., Erickson L., Davis D., Trogl J. “Recovering of soil from the military contaminated site in Sliac, Slovakia by Miscanthus x giganteus”. International multidisciplinary conference «Sustainable technologies and the legal/economic aspects of agricultural production», 27-28 April, 2016, Kyiv, Ukraine.

2. Stefanovska T., Kucherovska S. (presenting author), Lewis E., Pidlisnyuk V. Enthomocomplex of energetic plants for production of second generation biofuels.International multidisciolinary conference « Sustainable technologies and the legal/economic aspects of agricultural production», 27-28 April, 2016, NULES, Kyiv, Ukraine.

3. Nurzhanova A., “Physiological basis of the stability of miscanthus to soils polluted by heavy metals”.. International multidisciplinary conference «Sustainable technologies and the legal/economic aspects of agricultural production», 27-28 April, 2016, Kyiv, Ukraine.

4. Hettiarachchi G. ,“New Phytotechnology for Cleaning Contaminated Military Sites”, Annual Meeting of Multi-State Research Project (W3170: Beneficial Reuse of Residuals and Reclaimed Water: Impact on Soil Ecosystem and Human Health) funded by the United States Department of Agriculture- National Institute for Food and Agriculture (NIFA), June 26-28, 2016 at the Ohio State University, Columbus, Ohio, U.S.A.

5. Pidlisnyuk V., Stefanovska T., Trogl J., Shapoval P.,(presenting author), Erickson L. Biomass from second generation crops produced at the military contaminated land. International Symposium EPESSEM’2016: Environmental protection. Energy Saving. Sustainable environmental management”, Lviv, Ukraine, September 20-21, 2016.

6. Pidlisnyuk V., Stefanovska T., Kava L. . (presenting author) ,, Trögl J., Erickson L. Potential of biomass from Miscanthus x giganteus producing at the military contaminated land. International conference “Biomass for energy”, Section 1. “Resources of biomass and production”. Kyiv, Ukraine, September 20-21, 2016.

7. Pidlisnyuk V.(presenting author), Stefanovska T., Trogl J., Shapoval P. Developing perennial phytotechnology for contaminated military site: case of Kamenetz-Podilsky. International conference Contaminated Sites 2016, Bratislava, Slovakia, September 12-13, 2016.

8. Stefanovska T.(poster presenter) , Lewis E., Pidlisnyuk V., Kava L. “Changes of soil nematode community while planting miscanthusxgiganteus at the military contaminated sites” International conference Contaminated Sites 2016, Bratislava, Slovakia, September 12-13, 2016.

9. Valentina Pidlisnyuk presented project and research results at the Czech-Saxony Innovation Day was held in Prague, Czech Republic on September 10, 2016. Event was attended by Saxony Minister of Science and the Art Eva-Maria Stange and Vice-Prime Minister of Czech Republic on Innovation Pavel Belobradek (picture 1).

10. Valentina Pidlisnyuk presented project results at Dresden Tech Univerity, Germany, November, 2016 ( picture 2)

11. Lawrence Davis presented results of the project to an audience of about 50 in the Department of Biochemistry and Molecular Biophysics, Kansas State University on November, 30, 2016. An earlier version of this talk, including data from the Ft Riley project first year, was also given in the Department of Horticulture, Forestry and Recreation Resources at K-State on April 20, 2016.

http://military-site-cleaning.cz/wp-content/uploads/2017/03/picture-1-Pidisnyuk-report-at-Czech-Saxony-Innovation-Day-September-2016.jpg
Report of prof. Valentina Pidlisnyuk at Czech- Saxony Innovation Day, Czech Republic
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Introduction of NATO project at Drezden Tech University, Germany
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Report of Dr Ludmila Kava at the International conference “Biomass for energy”, Ukraine

”Phytotechnology with biomass production for restoration of contaminated and destroyed military sites” (Guide for training 1, in Ukrainian)