Joint research of UJEP, ZU, NULP
2019-year field experiment in Czech Republic and Lab experiment in Ukraine

On April 15 the re-establishing of the miscanthus field was done in Mimon, Czech Republic.   Altogether 900 rhizomes were equally planted at the contaminated site#1 and control site #2. Three different types of rhizomes were planted at the plantations: 300 rhyzomes were taken from the deposit field in St.Helena, Croatia; 300 rhizomes were taken from the research  field of the Czech Institute of Agricultural Research in Pruhonice and    300 rhizomes were purchased in Ukraine from the  Institute of the Sugar Beets and Energy crops.  The soil at both sites was preliminary treated by biochar produced by firm Agmeco LT, Czech Republic in order to evaluate the impact of that substance while miscanthus grows at the pure by nutrients soil. 

On April 12 the Lab experiment was established in Ukraine at the green house of the Ternopil Pedagogical University (TPU) in order to research the growing of miscanthus at the soil artificially contaminated by oil products. Effect of biochars to miscanthus growing at that soil and differences in uptake of oil products to miscanthus tissues will be jointly evaluated by research teams from NULP and TPU, the latest team joint the research within the bilateral agreement of cooperation with UJEP. That experiment will be served as modeling one of the military site contaminated by oil products.   Rhizomes for the experiment were taken at the deposit field in St. Helena, Croatia.
established plantation at site #2, Mimon
cleaning up the field, Mimon
digging up rhizomes at Pruhonice field
digging up rhyzomes
Dr. Kononchuk and Dr.Chomenchuk explained to students the design of the experiment
Dr.Herz is planting rhyzomes
established plantation at site#1, Mimon
PhD Diana Nebeska is planing rhizomes at site#1, Mimon
preparation of the field before planting
preparation of the field, Mimon
prof. Pidlisnyuk at the deposit field in St.Heena, Croaia
research team from TPU
rhyzomes' weighting before the planting, Lab experiment, TPU
treatment the soil by biochar

In July 2018 Ukrainian team have purchased a new piece of biosencoring equipment – three portative “Fluorotests” (Chlorophyll meters) for NATO SPS G4687 funds. The devices were constructed and manufactured at the Glushkova Institute of Cybernetics, National Academy of Sciences of Ukraine. The purchasing of those devices by NULP and NULES teams allowed researchers to expand objectives of ongoing project to chlorophyll fluorescence as a bioindicator of effects on growth M. x giganteus at contaminated sites for further biomass production. Chlorophyll-a fluorescence induction is a rapid technique for measuring photosynthetic electron transport in plants. Measuring of activity of M. x.giganteus grown at research military and deposit sites allows photosynthesis under stress to be studied in situ and in the laboratory. The concentration and fluorescence of chlorophyll has been measuring for M. x giganteus  in situ at Dolyna, Kurkhove military sites and Tokarivka PGR testing/deposit site.

Using of this technique will help to response to question how to mitigate the impact of stress factors while producing miscanthus biomass at contaminated sites.
NUBIP Young researcher Artem Medkow studies the effect of PGR to plant growth and development at Tokarivka
Measuring of M. x giganteus response to drought stress at Kurakhove site
NULP Young researcher Martyn Sozanskiy uses a new device to measure chlorophyll- a fluorescence at Dolyna site

Cooperation with liasoning institution Zagreb University and end-users from village St.Helena, Croatia

On May 2-9th, 2017 prof. Valentina Pidlisnyuk did a mission trip to Croatia. The goals of the mission were to discuss on-going research on M.xgiganteus growing at the contaminated land and to enrich the deposit Mxgiganteus plantation located at village St.Helena with partners from Agronomy Department at Zagreb University and potential end-uses of the phytotechnology.

On May, 3rd prof. Pidlisniuk met with Dr. Zeljka Zgorelec, ZU. Results of 2 years research on M.giganteus growing at the soil artificially contaminated by heavy metals and 2 years experiment with soil contaminated by metals taken from Bakar, Croatia were discussed. The design of training 2 scheduled at UJEP for November, 7-9 was observed and participation of ZU in this event was discussed. Dr.Zgorelec agreed to do lecturing on quality ensuring of soils and plants analysis. Prof. Pidlisnyuk met with Iva Hrelja, student from the Master program “Agroecology”, Antonija Jonjic and Ana Lijovic, students from Master program in “Organic farming and agrobusiness” and shared results of NATO SPS G4687 project.

During the visit prof.Pidlisnyuk updated on-going 3rd year semi-field experiment with M.xgigateus at the soil taken from Bakar and did sampling.

On May 4-5 the planting of additional deposit plantation at village st.Helena was done. The farmer organization passed the site for UJEP research on cost-sharing base and assisted in field plugging and planting process. The consultation with potential end-users- local farmers regarding planting of energetic crops at the Northern Croatia was done and questions about treatment of M.xgiganteus plantation against weeds was developed. The field was plugged, prepared for planting and 230 rhizomes of M.xgiganteus were planted. Currently the deposit plantation calculated 545 rhizomes. On May, 7 the fence from decorative bushes were planted in order to mark plantation.

On May, 8 prof. Pidlisnyuk made a field trip to Bakar, Rijeka region for additional sampling of metal- contaminated soil. Cost –sharing for that trip was done by potential end-users from village St.Helena.
Planting of M.xgiganteus at the deposit field
Meeting at the Agricultural Department, Zagreb University
Updating the 3rd year experiment
Field plugging
Preparation of the field
Assistance from the local end-users
Consultation with local farmers
Sampling at Bakar, Croatia

National University Lvivska Polytechnika (NULP)

Monitoring of the research plantation in Dolyna, Ukraine

On August, 30 a research team from NULP, Ukraine: Dr.Sc. Pavlo Shapoval, Co-Director and young researchers: Dr.Martyn Sozansky and Dr. Vitalyi Stadnik visited the research miscanthus plantation in Dolyna and did the regular monthly monitoring. The height of the plants, the number of shoots and the surviving rate were under investigation. They also measured the stress indicators using the shared with UJEP equipment FluorPen. Necessary maintaining was done and weeds were partly deleted manually. The necessary arrangement for the final measuring upon harvest was done as well. There are three different miscanthus plantations under the investigation in Dolyna research field, i.e.: 3 years’ plantations (contaminated-site 1; control-site 2) planted in 2017 while rhizomes were treated by PGRs: Stimpo, Regoplant, Charkor and 2 years’ plantation planted in 2018 (site 3) while different soil amendments (organic and mineral fertilizers, liming) were incorporate into the soil and rhizomes were treated by PGR Charkor.
Dr.Stadnyk while measuring plant's stress, site 3
Dr.Sozanskyi while measuring plant's bioparameters
Dr.Stadnyk while measuring bioparameters
site 1, 2017 planting
site 2, 2017 planting
site 3, 2018 planting

Establishing field plots at Dolyna, Ukraine

On April 3-5, 2017 the new research plots were established in Dolyna, Ivano-Frankivsk region, Ukraine. The place is former military site used as base of soviet army rockets and possess constantly a negative effect to the health of Dolyna habitats. The local authority is strongly aware of implementing the proposed phytotechnology for cleaning the site and production of biomass. Mayor of the city Volodymir Garazd participated in Training 1 in Lviv on October, 6-7, 2016 and initiated and promoted the establishing of that research Being involved in EU project on alternative energy and energy saving cities is implementing in Ukraine by Association “Energy Efficient Cities of Ukraine he proposed to use the site in Dolyna as demonstration one for governmental officials and professionals interested in alternative energy, including those involved in the EU project.

The establishing was jointly done by NULP and NULES in cooperation with new project’s partners from Ivan Franko Lviv National University and local administration of Dolyna city. Consequently, Co-Director Dr.Pavlo Shapoval, NULP led the establishing at the place assisted by PhD student Vytaliy Stadnik, NULP and PhD Student Artem Medkov, NULES. The design of the plots and scientific assistance was done by Dr.Volodymir Kvak, NULES and Dr. Lubov Byn’o, IFNUL. The local governmental officials: Ruslana Havrulkiv (Deputy Mayor of Dolyna city) and Andrij Yanishevskiy (Head of the Dolyna Soil Station) assisted in paper work and supplying the technical equipment for planting, which was a cost-sharing from the local authority.

Two plots (one - at the contaminated land and another - at the control land) were established with the size of 15,4 x22,1 m each, altogether 480 rhizomes were planted. Half of the M.x giganteus rhizomes were treated by PGRs “Stimpo” in order to increase the biomass quality and quantity.

Research at UJEP, Czech Republic
UJEP cooperation with the Crop Research Institute, Research Station in Chomutov

Two miscanthus fields were established in 2017 and 2018 years in cooperation with the Crop Research Institute (CRI), Research Station in Chomutov. Both fields are located at the marginal land belonged to CRI. The effect of PGRs to miscanthus production are monitored there along with the changing of the soil microbial cpommunities during multiyears miscanthus growth.

On July, 2020 Master student Ethan Duong from the University of Applied Science in Dresden, joint the UJEP team receiving a three month Erasmus scholarship from the Leonardo-Buro Sachsen, Germany. Ethan will assist in maintaining fields in Chomutov and Lab experiment related to impact of miscanthus rhyzomes’ microbization to the biomass quantity. The experiment was established by Dr. Kumar Pranaw within his PostDoc study at UJEP.
PostDoc Kumar Pranaw and Master student Ethan Doung discussed the maitaining of the experiment
Discussion at the Crop Researech Institute, July 1, 2020
Introductory meeting, from left to right student Ethan Doung, Dr.Ustak, prof. Pidlisnyuk,Dr.Pranaw

The UJEP miscanthus team meeting, May 15, 2019

On May 15, 2019 members of NATO team at UJEP: prof.Pidlisnyuk, young researchers Dr.Josef Trogl and Mgr.Hana Malinska, PhD student Diana Nebeska  had a regular working meeting on project research.  Two PostDocs affiliated with the project: Dr.Karim Al Souki and Dr.Kumar Pranaw  and  PhD student Hana Burdova participated in the  meeting along with visited professor Dr.Zeljka Zgorelec from the University of Zagreb, Croatia, liasoning institution of the project. 

Two main questions were discussed at the event, i.e.:

-evaluation of six-month study of Dr. Karim Al Souki while at the Post Doc at UJEP;

-plans for the joint research at the experiment facilities established in Dolyna, Ukraine and at the partner’s university in Ternopil, Ukraine.

Dr. Al Souki presented results of 6-month study related to NATO project while at PostDoc position at UJEP. Specifically, with assistance from the technical personnel from the Department of the Technical Sciences soil samples at the research miscanthus fields in Chomutov, Vrsebonice and Hradcany were collected and passed to partner’s university in Prague for measuring content of C. The first results of that monitoring will be presented at the regular NATO team meeting scheduled for June, 26.

With assistance of PhD student Hana Burdova and Ing. Jan Strodl the pot experiment with artificially contaminated by different concentrations of metals: Zn, Pb and Cd and oil products as well as their mixtures was established, Mxg originated from Zagreb was planted in that experiment. The monitoring of the plant’s parameters will be provided throughout the vegetation season, at harvest concentrations of the pollutants will be determined at the plant’s tissues. The 6-month studying report was approved with the caution that first manuscript on results based at the experiment done at UJEP will be prepared and submitted till December 1, 2019.

 At the second part of NATO working meeting the framework for joint research on stress measuring at the metals contaminated miscanthus site in Dolyna, and at the pot experiment with artificially oil-contaminated soils at Ternopil University was discussed.  The schedule for the soil sampling to be processed for the stress measuring at UJEP was developed.  Also plan for the preparation he manuscript based on the three years’ research experiment with soils from Bakar, Croatia was approved.
Dr.Zgorelec and Mgr. Malinska while at the evaluation
foreseen of the established experiment
members of the meeting at UJEP

The UJEP miscanthus team meeting

The UJEP miscanthus team meeting was held on January 18, 2019 in order to overview the state of the art with research fields in Czech Republic established within the project, i.e.:   Hradčany (visinity of Mimon), Chomutov and Všebořice. NDP Valentina Pidlisnyuk, members of UJEP NATO team:  Josef Trögl, Diana Nebeská, Hana Malinská and Jan Černý attended the event.  PhD student Hana Burdová, young researcher Jiri Štojdl and PostDoc Karim Al Souki partcipated in meeting as well. 

The  2018 data of  growing miscanthus  at three fields were discussed and perspective for research in 2019 was overviewed. In addition to existed data it is planned to accomplish the detailed analysis of the soil kharacteristics and contaminations  at  the research fields and to  present that data in a form of maps of the fields‘ characteristics. The monitoring of biomentic parameters during growing season and at harvest will be continued with requested picturing. In order to ensure the good growth of miscanthus in Mimon where the plantation will be re-established in spring 2019 is  was propose to use soil amendment-biochar, and to investigate the impact of its types (received after municipal waste treatment and after processing of biomass to energy) to miscanthus productivity.    

In addition the new research on  evaluation content of  C13/C12 in the soil  at   Chomutov and Všebořice miscanthus plantations at  3 depth has been initiated  by PostDoc Karim Al Souki in cooperation with PhD student Hana Burdová. The first results  will be reported to the team at the end of June, 2019.
discussion of results
discussion results of 2018 year
fields data
plans for 2019

New research fields established in the Northern Bohemia, Czech Republic

In spring 2018 two new  research fields  were established having the following research  goals:

  • Field testing of the impact of PGRs to M.xgiganteus biomass parameters
  • Monitoring of plant stress and it’s influence to biomass quality
  • Technological production of miscanthus biomass for further energy testing
  • Testing the phytotechnology with M.xgiganteus at the marginal land
Research field at Chomutov

On April 27, 2018 two plots was estabished at the research field of the Crop Research Institute, Chomutov. At the first plot rhizomes were planted according to the scheme used in 2017 (1 m x 1 m, 12 plants in the row). Half of the rhizomes were preliminary treated 12 hours before the planting by PGR Charkor with concentration 4 ml/l. Rhizomes treated by PGR were planted in every second row. The control plants were soaked into the clean water 12 hours before the planting and planted in each first row.

At the second research plot rhizomes were planted using the another scheme: 0,7 m x 0,5 m, 24 plants in row. That scheme is typical for establishing miscantus plantation and was used while establishing research fields in Ukraine. The order of rows with treated/untreated by PGR rhizomes was the same as in the first experiment.

All rhizomes planted at Chomutov were 2 years old and originally from the deposit site located at St.Elena, Croatia.
1_Charkor PGR
2_Treatment of rhizomes with PGR
3_Cultivation of field and Mr. Honzik from Chomutov Research institute
5_Small Miscanthus plant after 18 days
6_Miscanthus planted in 2017
7_Planting scheme in Chomutov

Research plots at Všebořice

On May 24th and 25th a research M.xgiganteus field was established at the former landfill located in Vseborice, Ústí nad Labem region (marginal land). The rhizomes used for planting were 10 years old and originally from the research plantation of The Silva Tarouca Research Institute of Landscape and Ornamental Gardening (RILOG), Pruhonice, Czech Republic.

The field was divided into the research plots, each of the plot was erected using the following scheme: 0,7 m x 0,5 m, 35 plants in the row, 6 rows; 210 rhizomes were planted in each unit.

Four of the research plots were established with rhizomes preliminary treated by PGRs. In two plots half of the planted rhizomes were treated by PGR Charkor with concentration 4 ml and time of exposure 12 hours. In two other plots half of the rhizomes were treated by PGR Stimpo with concentration 1 ml/l. Rhizomes treated by PGR were planted in every second row. The control plants were soaked 12 hours into the clean water before the planting and planted in each first row.

The rest of the plots established at the research field were planted using not treated rhizomes.Öhonice-which-was-used-for-rhizomes-preparation.jpg
1_Miscanthus in PrÖhonice which was used for rhizomes preparation
2_Cut Miscanthus stems
3_Consultation with Mr. Honzik
4_Digging of Miscanthus roots
5_Precleaning on field
6_Final cleaning of roots
7_Separating and cutting rhizomes
8_Rhizomes prepared for planting
9_Preparation of field by ploughing
10_The soil is very heterogenous
11_Planting in reclaimed dump
12_Finished fieldÁebo˝ice-dump.png
13_Planting scheme in VÁebo˝ice dump

Soil samples were taken from both localities in Chomutov and Všebořice for further nutrient analyses, detection of contaminants and determination of soil microorganisms.

Estabishing a new  greenhouse experiment  with simulated petroleum contaminated soil

The main goals of the greenhouse experiment are: to access the resistance of Miscanthusxgiganteus while growing at the simulated petroleum contaminated soil and to determine the limited level of soil petroleum contamination when the plant develops well and produces a good biomass.

The soil used for the experiment was taken at the petroleum refinery (Microchem company, Trebon, Czech Republic) and was polluted by different hydrocarbons. That soil was diluted with Mimon soil taken at the military site 1 (2016) which was not polluted by hydrocarbons. The different ratios of petroleum polluted-not polluted soils (0:1, 1:2, 1:1, 2:1, 1:0) were used for the experiment.

4 parallel pots with 6 kg of soil in each were prepared for each selected ratio. M.xgiganteus rhizomes were planted in 3 pots and one control pot was left without rhizomes. The rhizomes used for planting were originally from the same plant in order to ensure a genetic consistency and to avoid the effect of different stress tolerance.

The impact of soil petroleum contamination to plant’s biometric parameters, stress response and structure of soil microbial communities will be monitored during vegetation season.üany-airport.jpg
1_collecting of clean soil in Hradüany airport
2_clean soil and contaminated soil from previous experiment
3_prepared pots
4_Miscanthus plants after 17 days

Construction of fences at research sites in Hradčany airport (Mimoň)

On April 3-4, 2018 group of UJEP PhD students and employees visited research sites located at the former military airport Hradčany near Mimoň. They build a fence around both research plots where Miscanthus plants were planted in spring and autumn 2017. The reason was to protect them from herbivores which could damage biomass. It was a necessary step towards further successful Miscanthus field research. Fields were also marked with signs with information about the G4687 project and research activities.Áek-digging-hole-for-a-wooden-pale.jpg
PhD student M. DoÁek digging hole for a wooden paleÊtojdl-preparing-wooden-pales.jpg
PhD student J. Êtojdl preparing wooden pales
Work with netting
Fence around research plot
Finished fence
Sign with information about SPS G4687 project and Miscanthus research
UJEP employees and students

Enrichment of the research field plots in Mimon, Czech Republic

The research plots located in Mimon (contaminated and control) were enriched in November by planting 300 M.xgiganteus rhyzomes, from those 200 rhyzomes were planted at the contaminated site and 100 rhyzomes - at the control site. The experiment will permit to detect differences in growing the crop planted at the spring and at the fall as well as to research the peculiarities of soil microbial communities for both plantations established at the different seasons.

On October 31 both fields were prepared for planting by plugging. Preliminary the orientation plan of planting was created for both field and localities were measured for planting. Rhyzonmes were planted on November 7 (at the contaminated site) and on November 9 (at the control site) with assistance of participants of the Training 2.ří-Štojdl-UJEP-and-Chingiz-Nurzhanov-Al-Farabi-University-at-the-contaminated-site.jpg
Ing. Jiří Štojdl, UJEP and Chingiz Nurzhanov, Al-Farabi University at the contaminated siteří-Štojdl.jpg
measuring work at the contaminated site, prof. left to right PhDstudent Hana, prof. Pidlisnyuk, PhD student Diana Nebeska, Ing. Jiří Štojdl
PhD student Hana ,UJEP during measuring of the field
planting of rhyzomes at contaminated site by Dr.Kvak, Ukraine and Chingiz Nurzhanov, Kazakhstan
planting of rhyzomes by prof. Lawrence Davis
planting of rhyzomes, from lefy to right prof. Hettiarachchi, Dr.Stefanovska, Dr.Zgorelec, prof.Pidlisnyuk
preparation to planting at the control site
sampling at the contaminated site

Monitoring mission to the research sites at Czech Republic

On August, 17-18 prof. Pidlisnyuk and Trogl made a control mission to the research sites in Mimon (contaminated and control plots) and deposit site in Chomutov.

The both plots in Mimon show the good shooting rate of Miscanthus xgiganteus about 60%, and plantations are not much littering by weeds. The plantations were established in June because of delay in permission for planting and overcame the extremely hot temperature waves were in Czech Republic in July and August.

On August 18 prof. Pidlisnyuk and Trogl visited the deposit site planted at the Agricultural research station in Chomutov and met with Ing.Honzuk, head of department at the unit. The necessary work on maintaining plantation and cleaning it from weeds was discussed, also ways of treatment the MxG plantation by PGRs in 2018 was overviewed.
discussion the joint research on PGRs
First year plantation of MxG at Mimon (contaminated soil)
Former airport Hradcany, Mimon
Meeting at Chomutov agricultural station prof.Pidlisnyuk,, Ing.Honzik
monitoring of the control site,Mimon
monitoring of the deposit site, Chomutov
Mxg at the contaminated site, Mimon

New experiment with miscanthus growing at the oil-contaminated soil at UJEP

On July, 19 the new experiment was established at UJEP with growing miscanthus at the artificially oil-contaminated soil. The idea run from the fact that during sampling in spring 2017 one locality was found at the research site in Mimon contaminated by oil products. The source of the contamination was caused by previous release of oil-products into the soil by soviet troops while living airport in 90th. Such contaminated localities were cleaned up in 1996-2006 during implementation of projects supported by Czech Ministry of the Environment and other governmental agencies based at oxidation of oil-products inside the soil depth. However, the locality found is still remained contaminated.

The experiment has been designed in a way, that miscanthus seedling first produced at the regular agricultural soil during 2 month and further re-planted to the pots. The clean soil for the experiment was artificially contaminated by oil in the different proportions referred to the real concentration of contaminants at Mimon site. One seedling of miscanthus was planted in each pot which has 11 kg of differently contaminated soil.

The miscanthus growth will be monitored during the vegetation season and content of oil concentration will be measured.
Dr. Trogl and Erasmus student Gosterislioglu during soil samping in Mimon
Former military site at Mimon
Former storage of oil-products at Mimon
Planting of miscanthus seedling by PhD student Diana Nebeska
Soil sampling at the research site in Mimon on April 25

Field experiment in Hradčany airport – Mimoň, Czech Republic

Two experimental fields were settled in Hradčany former military airport near city Mimoň on June 1 2017. One locality is slightly contaminated with residuals of organic compounds as consequence of activities of Soviet army and the second one is control without hydrocarbon contamination.

Main goals of the experiment are:

  • Research of biomass production on military affected soil
  • Research of phytoremediation process
  • Research of influence of plant growth regulator on phytotechnology

Both localities were prepared by ploughing and 92 rhizomes were planted into each locality, half of them was treated by Stimpo plant growth regulator.
Members of UJEP team planting Miscanthus
Miscanthus rhizomes
Planting at control field
Preparation of field